Restless Legs Syndrome: Dopamine Dysregulation, Iron, and PLMS

Category: sleep-disorders Updated: 2026-02-27

RLS is characterized by urge to move legs at rest, with 80% of patients showing periodic limb movements during sleep (PLMS) every 20–40 seconds; ferritin below 50 ng/mL is linked to symptom severity and IV iron supplementation reduces PLMS by 70%.

Key Data Points
MeasureValueUnitNotes
Adult prevalence of RLS5–10% of populationHigher in women, older adults; clinically significant RLS ~2–3% of adults
PLMS prevalence in RLS patients80% of patientsPeriodic limb movements during sleep; ≥15/hour threshold for PLMS disorder
PLMS interval20–40seconds between movementsEach movement causes arousal; arousals accumulate to fragment sleep
Ferritin threshold associated with severity50ng/mLBelow this level, RLS symptoms worsen; below 20 ng/mL is high-risk
IV iron reduction in PLMS index70% reductionWang et al. 2013; IV ferric carboxymaltose; most effective in iron-deficient patients

Diagnostic Criteria (IRLSSG 2012)

Diagnosis requires all four criteria:

  1. Urge to move legs (or arms), usually accompanied by unpleasant sensations
  2. Symptoms worse at rest (lying, sitting)
  3. Partial or total relief with movement (walking, stretching)
  4. Circadian pattern: worse in the evening/night

Pathophysiology: Dopamine and Iron

The central model:

Iron deficiency (regional/brain) → Reduced dopamine synthesis → Reduced D2/D3 receptor activity in spinal cord and basal ganglia → Disinhibited sensorimotor processing → RLS symptoms

Evidence for this model:

  • CSF ferritin lower in RLS patients regardless of serum iron
  • Neuromelanin MRI shows reduced iron in substantia nigra in RLS
  • Dopamine agonists (pramipexole, ropinirole) are highly effective
  • IV iron supplementation produces sustained improvement

Periodic Limb Movements During Sleep (PLMS)

PLMS are stereotyped extensions of the big toe and dorsiflexion of the foot occurring every 20–40 seconds during NREM sleep. Each movement produces cortical arousal visible on EEG. A PLMS index (movements per hour) above 15 meets criteria for PLMS disorder.

Consequence: Sleep becomes fragmented, slow-wave sleep is disrupted, and patients wake unrefreshed. PLMS occurs without RLS in ~4% of adults and also in many other sleep disorders (sleep apnea, narcolepsy, REM behavior disorder).

Treatment

CategoryAgentsNotes
Dopamine agonistsPramipexole, ropinirole, rotigotineFirst-line pharmacotherapy; risk of augmentation
IronOral (with vitamin C) or IVFirst-line if ferritin <50 ng/mL
Alpha-2-delta ligandsGabapentin, pregabalinFirst-line if pain predominates
OpioidsLow-dose oxycodoneThird-line; severe refractory cases

Augmentation: A major complication of dopamine agonist therapy — symptoms spread to arms, worsen earlier in the day, become more severe. Risk increases with higher doses and longer treatment duration. Managed by switching to alpha-2-delta ligands or IV iron.

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Frequently Asked Questions

What is the connection between iron deficiency and restless legs?

Iron is a cofactor for tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. In RLS, brain iron deficiency — detectable in the substantia nigra and putamen via MRI or CSF ferritin — reduces dopaminergic tone in basal ganglia circuits that inhibit the spinal cord's sensorimotor system during rest. The result is disinhibited sensorimotor activity experienced as crawling, aching, or irresistible leg movement urges. Iron supplementation (oral if ferritin <50 ng/mL, IV if oral intolerant or severe) directly addresses the root mechanism and produces meaningful symptom improvement in many patients.

Why does RLS get worse at night?

RLS has a pronounced circadian pattern — symptoms peak in the evening and overnight, typically 10 PM–4 AM. This mirrors the circadian trough in dopaminergic activity and endogenous iron availability in the brain. Dopamine D2 receptor sensitivity oscillates across 24 hours, with lowest levels at night. The circadian system amplifies whatever underlying dopamine/iron deficiency exists. Additionally, the supine rest position typical of sleep onset maximizes sensory discomfort by eliminating the movement that temporarily relieves symptoms.

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