Caffeine and Sleep: Half-Life, Timing, and Sleep Architecture Effects

Category: environment-habits Updated: 2026-02-27

200mg caffeine taken 6h before bed reduces total sleep time by ~1 hour; caffeine half-life is 5–6h via CYP1A2; it blocks A1/A2A adenosine receptors, masking sleep pressure that rebounds upon its clearance.

Key Data Points
MeasureValueUnitNotes
Sleep loss from 200mg at 6h before bed~1hour total sleep reductionDrake 2013; subjects reported this was not disrupting sleep despite objective data
Caffeine half-life5–6hoursRange 3–10h; CYP1A2 enzyme; smokers faster (~3h), pregnant women slower (~9h)
Peak plasma caffeine30–45minutes after ingestionWell absorbed orally; peak plasma ~2–10 μg/mL from 200mg dose
SWS reduction from 200mg caffeineSignificantreductionLandolt 2004; slow-wave activity (SWA) in EEG reduced; sleep pressure masked
Standard coffee caffeine95mg per 240ml cupUSDA FoodData Central; range 72–130mg; espresso 63mg/30ml shot

Caffeine Pharmacokinetics

Caffeine is rapidly and almost completely absorbed after oral ingestion, reaching peak plasma concentrations within 30–45 minutes. It is distributed throughout total body water and crosses the blood-brain barrier freely.

Key pharmacokinetic parameters:

ParameterValue
Oral bioavailability~100%
Volume of distribution0.6 L/kg
Protein binding35%
Half-life (average)5–6 hours
Half-life (range)3–10 hours
Primary enzymeCYP1A2
Active metaboliteParaxanthine, theophylline, theobromine

CYP1A2 is highly polymorphic: “fast metabolizers” (about 50% of people, CYP1A2*1F allele) have half-lives of 3–4 hours; “slow metabolizers” have half-lives of 7–10+ hours. Pregnancy, oral contraceptives, and certain medications slow caffeine metabolism significantly.

The 6-Hour Effect

Drake et al. (2013) tested caffeine timing effects on sleep by giving subjects 400mg of caffeine at 0, 3, or 6 hours before bedtime. All three timing conditions significantly disrupted sleep compared to placebo — even the 6-hour condition reduced total sleep time by approximately 1 hour.

Crucially, subjects in the 6-hour caffeine condition reported not experiencing disrupted sleep despite objective polysomnographic evidence. This demonstrates the same subjective-objective dissociation seen in sleep deprivation research: people may not perceive the sleep disruption caused by caffeine but still experience it and its consequences.

Interaction with Adenosine

Caffeine blocks adenosine A1 and A2A receptors but does not affect adenosine production or accumulation. During the hours of caffeine action:

  • Adenosine continues to accumulate normally
  • Caffeine prevents adenosine from signaling sleep pressure
  • The “crash” occurs when caffeine is metabolized: accumulated adenosine suddenly gains access to all receptors simultaneously, producing intense sleepiness disproportionate to the expected level after caffeine’s duration

This dynamic explains why heavy caffeine users feel disproportionately tired when they stop drinking coffee — adenosine accumulation has continued during caffeine’s masked period, producing a withdrawal-like state.

For connection to other towers: see Coffee Tower caffeine-content page for caffeine levels by brew method, and Matcha Tower EGCG page for L-theanine’s interaction with caffeine.

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Frequently Asked Questions

What time should you stop drinking caffeine?

For a person with a typical 10pm bedtime and average 5.5h half-life, cutting caffeine at 1–2pm allows 8–9 hours for 75–80% of caffeine to clear. Many sleep researchers recommend a caffeine cutoff of noon–1pm for evening sleepers. Individual variation is large: fast CYP1A2 metabolizers can handle afternoon caffeine better; slow metabolizers (about 10% of people) may be affected by caffeine consumed in the morning.

Does caffeine affect deep sleep?

Yes. Even when caffeine doesn't prevent falling asleep, it reduces slow-wave activity (the EEG measure of deep sleep quality) during the night. Landolt et al. (2004) showed that 200mg caffeine taken in the morning measurably reduced SWA during that night's sleep — demonstrating that caffeine effects persist far beyond waking alertness and impair the restorative quality of sleep even when total sleep time seems normal.

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